Invitrogen

Butyrylcholinesterase Monoclonal Antibody (3E8)

Butyrylcholinesterase Antibody in ELISA (ELISA)

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Butyrylcholinesterase Antibody (HAH 002-01-02) in ELISA

Sandwich ELISA of Butyrylcholinesterase using HAH 002-01 as the capture antibody and HAH 002-01B as the biotinylated detection antibody.

Butyrylcholinesterase Antibody in Western Blot (WB)

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产品信息

HAH 002-01-02

应用

建议稀释比

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免疫印迹 (WB)

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酶联免疫吸附实验 (ELISA)

1:20,000

免疫沉淀 (IP)

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中和实验 (Neu)

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亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

3E8

抗原

Butyrylcholinesterase isolated from human plasma.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.05% sodium azide

保存条件

-20° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2063729

产品详细信息

HAH 002-01-02 has specificity for butyrylcholinesterase from human serum or plasma. Crossreactivity to BtChE from other species has not been tested.

HAH 002-01-02 has been successfully used in ELISA techniques.

HAH 002-01-02 reacts with BtChE from normal serum when tested in sandwich ELISA. This antibody can be used as both the capture and detection antibody or an alternative antibody against BtChE can be used as capture antibody. In addition, it can be used in crossed immunoelectrophoresis followed by incubation of the gel with HAH 002-01-02. In immunoblotting after SDS-PAGE no reaction is seen with either the reduced or with the non-reduced form of BtChE. No reaction is seen with acetylcholinesterase from human nervous tissue and erythrocytes.

The HAH 002-01-02 immunogen is Butyrylcholinesterase found in human plasma cells.

靶标信息

Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Simultaneous Time-concentration Analysis of Soman and VX Adducts to Butyrylcholinesterase and Albumin by LC-MS-MS.

Journal of analytical toxicology

Lee JY,Kim C,Lee YH

HAH 002-01-02 was used in Affinity Purification to determin that VX and GD-tyrosine is more suitable than VX and GD-nonapeptide as a biomarker owing to its longevity.

Fri Jun 01 00:00:00 EDT 2018

Comparison of Efficiency of Purification (from Human Plasma) of a Nerve Agent Adduct of Butyrylcholinesterase Between the Affinity Gel Method and Immunomagnetic Separation.

Journal of chromatographic science

Lee JY

HAH 002-01-02 was used in Immunoprecipitation to purification efficiency between the procainamide affinity gel method and immunomagnetic separation for the nerve agent adduct of BChE in plasma and then optimized the sample preparation by purifying BChE to measure biomarkers of human exposure to organophosphorus nerve agents.

Thu Mar 01 00:00:00 EST 2018

Monoclonal antibodies to human butyrylcholinesterase reactive with butyrylcholinesterase in animal plasma.

Chemico-biological interactions

Peng H,Brimijoin S,Hrabovska A,Krejci E,Blake TA,Johnson RC,Masson P,Lockridge O

HAH 002-01-02 was used to research monoclonal antibodies to human butyrylcholinesterase and their reaction in animal plasma with butyrylcholinesterase

Tue Jan 05 00:00:00 EST 2016

Comparison of 5 monoclonal antibodies for immunopurification of human butyrylcholinesterase on Dynabeads: KD values, binding pairs, and amino acid sequences.

Chemico-biological interactions

Peng H,Brimijoin S,Hrabovska A,Targosova K,Krejci E,Blake TA,Johnson RC,Masson P,Lockridge O

HAH 002-01-02 was used in immunoprecipitation to study monoclonal antibodies that recognize human butyrylcholinesterase

Mon Oct 05 00:00:00 EDT 2015

Detection of cresyl phosphate-modified butyrylcholinesterase in human plasma for chemical exposure associated with aerotoxic syndrome.

Analytical biochemistry

Schopfer LM,Masson P,Lamourette P,Simon S,Lockridge O

HAH 002-01-02 was used in Immunopurification to develop a mass spectrometry method for detection of the cresyl phosphate adduct on human butyrylcholinesterase as an indicator of exposure.

Mon Sep 15 00:00:00 EDT 2014

A high-throughput diagnostic method for measuring human exposure to organophosphorus nerve agents.

Analytical chemistry

Knaack JS,Zhou Y,Abney CW,Jacob JT,Prezioso SM,Hardy K,Lemire SW,Thomas J,Johnson RC

HAH 002-01-02 was used in blocking or activating experiment to develop an automated high throughput method for diagnosing exposure to organophosphorus nerve agents

Tue Nov 06 00:00:00 EST 2012

Immunomagnetic separation and quantification of butyrylcholinesterase nerve agent adducts in human serum.

Analytical chemistry

Sporty JL,Lemire SW,Jakubowski EM,Renner JA,Evans RA,Williams RF,Schmidt JG,van der Schans MJ,Noort D,Johnson RC

HAH 002-01-02 was used in immunoprecipitation to develop a new method to extract butyrylcholinesterase from serum for detecting nerve agent exposure

Sun Aug 01 00:00:00 EDT 2010

生物信息学

蛋白别名: Acylcholine acylhydrolase; BtChE; Butyrylcholine esterase; Choline esterase II; Cholinesterase; cholinesterase (serum) 2; cholinesterase 1; EC 3.1.1.8; Pseudocholinesterase

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基因别名: BCHE; CHE1; CHE2; E1

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UniProt ID: (Human) P06276

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Entrez Gene ID: (Human) 590

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功能

beta-amyloid binding catalytic activity acetylcholinesterase activity cholinesterase activity hydrolase activity, acting on ester bonds enzyme binding choline binding identical protein binding esterase

参与通路

learning negative regulation of cell proliferation neuroblast differentiation choline metabolic process response to drug response to alkaloid cocaine metabolic process negative regulation of synaptic transmission response to glucocorticoid response to folic acid